Materials and methods: The anti-inflammatory activity of MGEB was evaluated using 2,4-dinitrofluorobenzene (DNFB)-induced allergic contact dermatitis (ACD) in mice.
glyptostroboides, in this study, we evaluated the anti-inflammatory activities of MGEB on 2,4-dinitrofluorobenzene (DNFB)-induced mouse model of ACD, then further investigated its potential mechanisms by determining its inhibition on T cell activation and Th1/Th17 responses in vitro.
The contact sensitizers 2,4,6-trinitrobenzene-sulfonic acid (TNBS), 2,4-dinitrofluorobenzene
(DNFB; Sigma) in 4:1 AOO, onto the dorsum of the ears for 3 consecutive days.
After alkalinization, supernatants were reacted with 2,4-dinitrofluorobenzene
, acidified, and analyzed by HPLC (Agilent series 1100) (10).
Methods: We treated NJJ on 2,4-dinitrofluorobenzene
(DNFB)-induced AD-like skin lesions in NC/Nga mice, phorbol myristate acetate/calcium ionophore A23187-stimulated human mast cell line (HMC-1) cells, and anti-CD3/anti-CD28-stimulated splenocytes.