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2,4-dinitrophenylhydrazine

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2,4-dinitrophenylhydrazine [¦tü ¦fȯr dī¦nī·trō‚fen·əl′hī·drə‚zēn]
(organic chemistry)
(NO2)2C6H3-NHNH2A red, crystalline powder with a melting point of approximately 200°C; soluble in dilute inorganic acids; used as a reagent for determination of ketones and aldehydes.


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2'-7'-dichlorofluorescein diacetate (DCFH-DA), 2'-7'-dichlorofluorescein (DCF), trichloroacetic acid (TCA), 2,4-dinitrophenylhydrazine (DNPH), guanidine hydrochloride, phenylmethylsulfonyl fluoride (PMSF), 5-amino-2,3-dihydro-l,4-phtalazinedione (luminol) and hydrogen peroxide stock solution were purchased from Sigma Chemical Co.
With respect to the used base concentration, the developed spectrophotometric procedure with p-DMAB benzaldehyde derivative was found to be more practically appropriate than the modified Randle reference method, which is a very common used spectrophotometric method for the analysis of pyruvic acid in onions via the utilization of 2,4-dinitrophenylhydrazine (DNPH) color agent [35].
Briefly, 10 [micro]L mitochondrial protein was derivatized with 2,4-dinitrophenylhydrazine and blotted to polyvinylidene fluoride membrane (Amersham Biosciences, Piscataway, NJ).
 
 
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