gentisic acid

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gentisic acid

[jen′tis·ik ′as·əd]
(organic chemistry)
C7H6O4 A crystalline compound that forms monoclinic prisms from a water solution, sublimes at 200°C, melts at 250°C, and is soluble in water, alcohol, ether, sodium, and salt; used in medicine. Also known as gentianic acid.
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The matrix consisted of 100 g/L 2,5-dihydroxybenzoic acid (DHB) (Fluka) in a solution consisting of 1 volume of acetonitrile and 1 volume of 20 mmol/L aqueous sodium acetate.
We purchased the matrix, 2,5-dihydroxybenzoic acid (DHB), trifluoroethanol, sodium hydroxide, CHAPS, Tris-HCl, sodium pyrophosphate, EDTA, and EGTA from Aldrich; chloroform, iodomethane, and sodium chloride from EM Science; dithiothreitol (DTT) and iodoacetamide (IAA) from Bio-Rad Laboratories; ammonium bicarbonate from Mallinckrodt Chemical Company; and acetonitrile from Fisher Scientific.
SA was selected as the MALDI-TOF MS matrix of choice for this investigation because other standard matrices used in protein analysis, [alpha]-cyano-4-hydoxycinnamic acid and sDHB [a 9:1 (by weight) mixture of 2,5-dihydroxybenzoic acid and 5-methoxysalicylic acid], did not yield intense spectra for hCG[beta]cf (data not shown).

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