mercaptoethanol

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mercaptoethanol

[mər‚kap·tō′eth·ə‚nȯl]
(organic chemistry)
HSCH2CH2OH Mobile liquid, water-white; soluble in water, benzene, ether, and most organic solvents; boils at 157°C; used as a solvent, chemical intermediate, and reducing agent.
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1 mM 2-mercaptoethanol (Gibco, USA), 1% non-essential amino acids (Gibco, USA), 2 mM L-glutamine (Gibco, USA), 1 mM MEM sodium pyruvate solution (Gibco, USA), 1% penicillin/streptomycin (Gibco, USA), and 1,000 U/mL LIF (ES-GRO, Chemicon, Billerica, MA, USA).
1g fresh leaf tissue in 4 M guanidium thiocyanate (Sigma, 59980) buffer mixed with 2-mercaptoethanol (Sigma, M3148) at a ratio of 1:125 (2-mercaptoethanol to guanidium) using a sterile mortar and pestle followed by the triplicate RT-PCR performed in a one-step reaction using superscript [TM] III RT/Platinum[R] Taq Mix System (Invitrogen Life Technologies) in a GeneAmp PCR system 9700 (Applied Biosystems, UK) as per Rwegasira [25].
1 To 16):- 2-Mercaptoethanol, Make: Biorad Or Equivalent, Cat No.
We cultured human Jurkat T cell lines in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum, penicillin (100 U/mL), streptomycin (100 jug/mL), 10 mmol/L HEPES, 2 mmol/L L-glutamine, and 25 [micro]mol/L 2-mercaptoethanol at 37[degrees]C in a humidified 5% C[O.
In this research, zinc sulfide quantum dots were synthesized in forms of pure and doped with iron ion with mean size of 1-2 nm through chemical deposition method in the presence of 2-mercaptoethanol as a capping agent.
01% Triton X-100 (Sigma-Aldrich, USA), 1% polyvinylpolypyrrolido-ne (Sigma Aldrich), 1 mM EDTA (Sigma-Aldrich), 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) and 1 mM 2-mercaptoethanol (Sigma-Aldrich).
5] M 2-mercaptoethanol, minimal essential medium (MEM) vitamin solution, MEM nonessential amino acid solution (all form Invitrogen), [10.
The final precipitates were dissolved in 1 ml buffer containing 8 M urea and 5% 2-mercaptoethanol.

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