1 mM 2-mercaptoethanol
(Gibco, USA), 1% non-essential amino acids (Gibco, USA), 2 mM L-glutamine (Gibco, USA), 1 mM MEM sodium pyruvate solution (Gibco, USA), 1% penicillin/streptomycin (Gibco, USA), and 1,000 U/mL LIF (ES-GRO, Chemicon, Billerica, MA, USA).
1g fresh leaf tissue in 4 M guanidium thiocyanate (Sigma, 59980) buffer mixed with 2-mercaptoethanol
(Sigma, M3148) at a ratio of 1:125 (2-mercaptoethanol
to guanidium) using a sterile mortar and pestle followed by the triplicate RT-PCR performed in a one-step reaction using superscript [TM] III RT/Platinum[R] Taq Mix System (Invitrogen Life Technologies) in a GeneAmp PCR system 9700 (Applied Biosystems, UK) as per Rwegasira .
SDS-PAGE was carried out using a 10-15% (w/v) gradient slab gel in the presence or absence of 2-mercaptoethanol
1% of 2-mercaptoethanol
using a sterile micropestle in 1.
1 To 16):- 2-Mercaptoethanol
, Make: Biorad Or Equivalent, Cat No.
We cultured human Jurkat T cell lines in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum, penicillin (100 U/mL), streptomycin (100 jug/mL), 10 mmol/L HEPES, 2 mmol/L L-glutamine, and 25 [micro]mol/L 2-mercaptoethanol
at 37[degrees]C in a humidified 5% C[O.
In this research, zinc sulfide quantum dots were synthesized in forms of pure and doped with iron ion with mean size of 1-2 nm through chemical deposition method in the presence of 2-mercaptoethanol
as a capping agent.
01% Triton X-100 (Sigma-Aldrich, USA), 1% polyvinylpolypyrrolido-ne (Sigma Aldrich), 1 mM EDTA (Sigma-Aldrich), 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) and 1 mM 2-mercaptoethanol
5 mM 2-mercaptoethanol
in 250 mM potassium phosphate at pH 7.
5] M 2-mercaptoethanol
, minimal essential medium (MEM) vitamin solution, MEM nonessential amino acid solution (all form Invitrogen), [10.
5 mL methanol and 3 mL 2-mercaptoethanol
and made up to 560 mL using 0.
The final precipitates were dissolved in 1 ml buffer containing 8 M urea and 5% 2-mercaptoethanol