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4-nitrophenol, 2-aminobenzoic acid, benzamide, 4-aminobenzoic acid, resorcinol, obromonitrobenzene and 2-nitroaniline were used as active aromatic coupling compounds for the second step.
Starting from available 2-nitroaniline, we accessed 3,8-dibromobenzo[c]cinnoline through a coupling reaction and a ring-forming diazotization.
Vitamin C content was determined using the colorimetric method of the 2-nitroaniline (Bernal, 1998).