para-nitroaniline

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para-nitroaniline

[¦par·ə ‚nī·trō′an·ə·lən]
(organic chemistry)
NO2C6H4NH2 Yellow crystals that melt at 148°C; insoluble in water, soluble in ethanol; a toxic material; used to make dyes, and as a corrosion inhibitor.
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One unit of trypsin activity corresponded to 1 [micro]mol 4-nitroaniline liberated in 1 min/mL extracellular enzymatic extract, based on the extinction coefficient of the substrate ([epsilon]407 = 8,200/M/cm).
Biodegradation products of Acid Orange 7 and Disperse Orange 3, by white rot fungus, Pleurotus ostreatus, were identified as 4-hydroxybenzenesulfonic acid, benzenesulfonic acid, 1,2-naphthoquinone, 4-nitrophenol, nitrobenzene, 4-nitroanisole, and 4-nitroaniline.
It describes the use of [gamma]-glutamyl-4nitroanilide as a substrate that is cleaved to form the easily measured 4-nitroaniline.
The precision, in terms of CV of the absorbance of twelve repetitive recordings (390 nm, 150 [micro]L of buffered solutions of 4-nitroaniline per well), was [+ or -]0.
The liberation of 4-nitroaniline was observed at 390 nm for at least 15 min with a sampling rate of 120 data points per min.
Because of the peculiarities of the coupled assay, the remaining part contributes to the unwanted background absorbance attributed to 4-nitroaniline at 390 nm [15,24,25].