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Methods: Human limbal tissues obtained from the cadaveric donor eyes were cultured over the denuded human amniotic membrane in the presence of mitomycin C treated 3T3 fibroblasts and the cultured cells studied for the expression of ABCG2 and p63 by immunohistochemistry and Western blot.
Melanoma contains CD133 and ABCG2 positive cells with enhanced tumorigenic potential.
Further characterization of these haplotypes using sequence information, radiation hybrid mapping and comparative mapping with the human genome sequence resolved the QTL to a 420Kb haplotype region between gene ABCG2 and LAP3 (Olsen et al.
An early loss of NSC self-renewal gene expression (p63, ABCG2, BMI-1, SHH, OCT-4, NOTCH-1) during arsenite exposure was subsequently reversed as the tumor suppressor gene PTEN was progressively suppressed and the CSC-like phenotype acquired.
This study was carried out to evaluate and compare the expression of the stem cell associated marker: ABCG2, keratinocyte stem cell marker: p63 and corneal differentiation markers: Cnx43 and K3/K12 on limbal explants cultured on human amniotic membrane (HAM) with intact epithelium and HAM denuded of its epithelium.
This study is the first to show that a specific mutation in the pump, called ABCG2, is associated with a drug-induced side effect, according to researchers.
Although the differentiation-dependent and sterol-regulated induction of ABCA1 and ABCG1 is well established (7), parallel transcript profiling, using our Human ABC Transporter TaqMan Low-Density Array, revealed several additional differentiation-dependent ABC transporters and novel LXR/RXR-regulated ABC transporters, including ABCB1 (MDR1), ABCB9, ABCB11 (BSEP), ABCC2 (MRP2), ABCC5 (MRP5), ABCD1 (ALD), ABCD4, and ABCG2.
91-93) Genes activated by HIF-2[alpha] alone include the stem cell factor gene Oct4 (94) and ABCG2.
Based on the iHS method, specific haplotype frequencies in the genomic regions containing MSTN (relevant to muscle development), ABCG2 (relevant to milk yield and composition) and KHDRBS3 (relevant to intra-muscular fatness) might have resulted from selective sweeps.
This mutation gives ABCG2 the ability to transport a wide variety of anticancer drugs from the anti-folate family outside of the malignant cell.
The correlation coefficients for the calibration curves of ABCG2 and GAPDH (Fig.
A key finding from this 14-year population-based study of older adults was that a variant in the ABCG2 gene was associated with a 46 percent increased risk for ischemic stroke in Caucasian and African American participants.
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