It was observed that the addition of taurine (20 mM) and manganese (150 uM) individually improved the post-thaw sperm motility, viability, membrane integrity, and acrosome
Semen at pre-freeze and frozen-thaw phase was analyzed for sperm function (motility, viability and Hypo osmotic swelling test [HOST]), acrosome
reaction and lipid peroxidation [LPO]) as detailed below.
is an acidified organelle that can be specifically stained by LysoTracker probes, which spontaneously enter acrosome
sup], Round-headed sperm cannot fertilize mature oocytes because of the absence of acrosome
and acrosomal enzymes.
integrity was assessed by fixation with 2% glutaraldehyde in BTS, the number of sperm with normal apical ridge was recorded after counting 200 spermatozoa in a 400X magnification phase contrast microscope (GADEA et al.
Giemsa accumulates in spermatozoa with an intact acrosome
, staining the acrosome
region in purple.
The majority of semen samples (>90%) of all populations were categorized as live with intact acrosomes
(Category 1) and there was no significant difference among populations (Kruskal-Wallis test, H = 3.
reaction is one of the most important events that occur prior to fertilization.
Species with external fertilization had small sperm (usually <70-[micro]m long) with a simple morphology (head with sub-spherical nucleus and caplike acrosome
, midpiece with a small number of unmodified mitochondria surrounding the two centrioles.
The present study was conducted to observe the effect of royal jelly (R J) on motility, viability and acrosome
integrity of spermatozoa during post-thaw incubation.
The sperm binds to the ZP and undergoes the acrosome
reaction, releasing enzymes that allow penetration of zona pellucida.
Glutaraldehyde fixations of boar spermatozoa for acrosome