staining assay by fluorescein isothiocyanate-Pisum sativum agglutinin
The slides were observed under a light microscope (Olympus BX60) at approximately 1000 x and assessed for the number of sperm that had undergone acrosome
75, the acrosome
should cover the anterior head and should comprise 40% to 70% of the head area.
During the fertilization process, specific protein-degrading enzymes are added to the acrosomal membrane of spermatozoa in the epididymis, and spermatozoa are capacitated by the uterine environment and hormones, thereby completing the acrosome
reaction (Phelps et al.
The fucose sulfate polymer of egg jelly binds to sperm REJ and is the inducer of the sea urchin sperm acrosome
76%) and rate of morphologically normal acrosome
Labeling by LcH and PNA differed slightly in partially reacted sperm relative to fully opened acrosomes
gigas, sperm acrosomes
were morphologically damaged at higher UV intensities (Li et al.
of a Octopus tankahkeei spermatozoon is a 5-[micro]m long helical structure that resembles an aiguille in shape (Fig.
We also found that several granules of high electron concentration existed in the acrosomes
of frozen-thawed spermatozoa.
This agglutination is likely due to spontaneous acrosome
reactions which could have occurred at some point during freezing or thawing.
Ejaculates were evaluated for volume, mass activity (undiluted semen), consistency (density judged subjectively), motility (diluted with normal saline), progressive motility, live spermatozoa (eosinnigrosin stain), normal acrosomes
(by viewing wet mounts of diluted spermatozoa fixed in buffered Formalin-Citrate solution) and sperm concentration (sperm cells were counted in four squares of a hemocytometer after 1:200 dilution of semen with 0.