zwitterion

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Related to Ampholytes: isoelectric point, zwitterion

zwitterion

[′tsfid·ər‚ī‚än]
(chemistry)
References in periodicals archive ?
01[micro]M ortho phosphoric acid as anolyte, 8M urea and 250[micro]l of ampholyte as sample overlay.
DNA) and low-molecular-weight ampholytes and strong protein associations.
9% (by volume) pH 3-10 ampholytes (SERVALYT[R], SERVA).
Each solution was then dried by centrifugation under reduced pressure, after which 180 =L of rehydration buffer (7 mol/L urea, 2 mol/L thiourea, 20 g/L CHAPS, 50 mmol dithiothreitol, and 5 mL/L ampholytes, pH 3-10) was added to the dry sample to solubilize and denature the proteins.
Each serum sample (500 [micro]g of protein) was solubilized in 8 mol/L urea, 40 mL/L CHAPS, 40 mmol/L Tris, 2 mL/L Bio-Lyte 3/10 ampholytes (Sequential Extraction Buffer 2; Bio-Rad) with 5 mmol/L tris(2-carboxyethyl)phosphine reducing agent and incubated for 1 h at room temperature.
variability in gel conductivity, quality of carrier ampholytes, and electroendosmosis) make IEF inherently difficult to standardize.
In addition, sequestering of iron by the ampholytes during IEF might also occur.