zwitterion

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Related to Ampholytes: isoelectric point, zwitterion

zwitterion

[′tsfid·ər‚ī‚än]
(chemistry)
References in periodicals archive ?
4% (v/v) carrier ampholytes (pH 3-10, BioRad)] was loaded onto a 7 cm, pH 3-10 immobilised pH gradient strip (IPG, BioRad, USA).
we can assert that synthesized polymeric ampholyte contains in the structure except amino groups also phosphate groups.
01[micro]M ortho phosphoric acid as anolyte, 8M urea and 250[micro]l of ampholyte as sample overlay.
Proteins are ampholytes, exhibiting amphoteric behavior owing to the presence of both positively- and negatively-charged side chain groups distributed along the length of the molecule and at its ends.
0 ml ampholytes (pH gradient 4-5; Crescent Chemical, Hauppa, NY), 4.
DNA) and low-molecular-weight ampholytes and strong protein associations.
9% (by volume) pH 3-10 ampholytes (SERVALYT[R], SERVA).
5; marine byssal precursors, in contrast, are highly basic - many with pIs exceeding the effective resolving range of available ampholytes.
In vitro-labeled samples were focused with wide range ampholytes (pH = 3-10; Bio-Lyte 3/10, Bio-Rad) in 4 M urea and 10% NP-40, and size-fractionated on 10% sodium-dodecyl-sulfate poly-acrylamide gels (SDS-PAGE) along with Brome Mosaic Virus (BMV) molecular weight markers (110, 97, 35 and 20 Kd) provided as a control with in vitro translation kits (Promega).
Each solution was then dried by centrifugation under reduced pressure, after which 180 =L of rehydration buffer (7 mol/L urea, 2 mol/L thiourea, 20 g/L CHAPS, 50 mmol dithiothreitol, and 5 mL/L ampholytes, pH 3-10) was added to the dry sample to solubilize and denature the proteins.