Kohler illumination

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Kohler illumination

[′kō·lər i‚lü·mə′nā·shən]
(optics)
A method of illumination for the optical microscope used with coiled filaments or other sources of irregular form or brightness; an image of the filament large enough to fill the iris opening is focused on the condenser which is focused so that the image of the iris diaphragm on the lamp is in focus with the specimen, and the lamp iris is opened only enough to fill the field of view; the iris of the microscope is opened only enough to illuminate the back aperture of the objective; no ground glass is used.