However, this is unlikely, as the requisite molecular components for the apoptotic cascade are available in the blastomeres
of embryos at all stages of development (Weil et al.
Improved embryo development with decreased apoptosis in blastomeres
after the treatment of cloned bovine embryos with [beta]-mercaptoethanol and Hemoglobin.
The separated blastomeres
were coated with an artificial zona pellucida and placed in the culture medium used for in vitro fertilization (IVF).
PCR is a rapid, easy procedure for large scale sexing, and primers derived from many Y-specific sequences have been used to screen blood, meat and blastomere
samples (Zeleny et al.
The procedure, known as "Accurate 23 chromosome aneuploidy screening in human blastomeres
using single nucleotide polymorphisms (SNP)," makes it possible for the first time to fully evaluate the genetic make up of human embryos quickly and accurately enough to use that information to help improve pregnancy rates, reduce miscarriage risk, and decrease the risk of major congenital anomalies such as Down syndrome for patients treated with IVF.
First proposal: "Pluripotent human stem cells are to be derived from early IVF embryos (roughly 4-8 cells) that have spontaneously died (as evidenced by the irreversible cessation of cell division) but some of whose blastomeres
appear normal and healthy.
103) After twelve hours, twenty of the twenty-two eggs (ninety percent) had begun the process of cell division; after five days, six of the original twenty-two (thirty percent) had divided to the point of forming blastomeres
Embryos were considered normal if blastomeres
of equal size were present within the boundaries of the vitelline membrane.
FISH analysis (fluorescent in situ hybridization) was performed on 80% of the biopsied blastomeres
His later identification of blastomeres
with organs is also problematic.
At that time, researchers knew that the egg starts to divide after fertilization, forming genetically identical cells, or blastomeres
The full article, "Preclinical validation of a microarray method for full molecular karyotyping of blastomeres
in a 24-h protocol", by Johnson et.