Flavonolignans were added to cell lysate
at final concentrations of 5-100 [micro]M and incubated for 10 min at room temperature before addition of NADPH.
05), the cutoff of the reaction was determined for each probe as two times the mean value of HPV-negative TF-1 cell lysate
4 [micro]M F6/F4, 80 [micro]M PCR-grade dNTPs (Roche Molecular Biochemicals), 2 [micro]L of dimethyl sulfoxide (Merck), and 1 [micro]L of thawed cell lysate
Based on the company's proven amplification technology, the WT-Ovation[TM] One-Direct RNA Amplification System uses a simple, three-step process to prepare samples directly from cell lysate
for analysis using microarrays or qPCR.
The kits offer the ULS labeling molecule bound to Biotin and Fluorescein for direct labeling of protein analytes in serum and cell lysate
6-Mercaptopurine (6-MP; 10 [micro]L; final concentration, 4 mmol/L) was added to 300 [micro]L of red cell lysates
containing 100 [micro]L of 0.
For determination of total IL-8, a blood cell lysate
was prepared from each EDTA blood sample: an 0.
This early cell lysate
work in the VEGF receptor family indicates the potentially broader reach of our technology, where receptor activation through protein complex formation is a key feature of the biology.
We propose a streamlined RNA isolation method where poly(A)+ mRNA is isolated directly from the cell lysate
by binding to an oligo(dT)-coated manifold support.
PREPARATION OF BLOOD CELL LYSATES FROM RAT AND HUMAN BLOOD
Our goal in this review was to summarize recent advances in protein and cell lysate
arrays while providing real-world examples of their usage," says John Austin, contributing author, Executive VP and CSO at Aushon BioSystems.
Because cell lysate
contained mRNA that had Alu sequences, the emission in rlu of the Alu sequence in cell lysate
might be higher than that in purified genomic DNA.