In cryosections, naphthyl AS-D chloroacetate
and Tween-80 did not yield any colorimetric reactions at any of the three pHs, whereas [alpha]-naphthyl acetate yielded strongly positive results at pH 7.
The pH-independent hydrolysis of 4-nitrophenyl chloroacetate
(4-NPCA) was used as a model reaction (Scheme 1) to test the online spectrophotometric system for ultrasonic kinetic measurements.
In order to explain these results, the Quantum Theory of Atoms in Molecules is used to examine the conformational behavior of the chloroacetate
anion at the atomic level.
In this report we have set out a broader investigation into hydrophobic effects of aliphatic alcohols as co-solvents on ester hydrolysis in water, and studied the neutral hydrolysis of 4-nitrophenyl chloroacetate
in the presence of small amounts of alcohols.
They were negative for myeloperoxidase (MPO), for chloroacetate
esterase (CAE) and for all other myeloid and T-cell markers.
Reacting the amines with sodium chloroacetate
produces betaines, another surfactant found in shampoos.
3) at least one member of the group consisting of units derived from unsaturated carboxylic acids, alkyl esters of unsaturated carboxylic acids, alkoxy alkyl acrylates, allyl glycidyl ether, vinyl chloroacetate
, ethylene, butene-1 and isobutylene and units resulting from the hydrogenation of units from a conjugated diene.
Firstly, under microwave irradiation and phase-transfer catalysis conditions, these phenols reacted with methyl chloroacetate
to synthesize substituted phenoxyacetates that were then hydrolyzed to get the corresponding phenoxyacetic acid compounds (a1-a5) according to the reported procedure.
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Five tumors (29%) were assessed with naphthol-ASD chloroacetate
After releasing their woundhealing granules, mast cells lose the ability to make the enzyme chloroacetate
esterase and no longer show up when exposed to a dye that reacts with the enzyme.
Cytospins (Shandon Cytospin 3) of freshly collected hemocytes ([less than or equal to] 50-100 [micro]1) from individuals with hemolymph that was normal (0% CCH), fully cancerous (100% CCH), or from CCH maintained in vitro were treated with the following stains and enzyme assays normally used to characterize human blood cells: Romanovsky stain (Thermo-Shandon, Kwik Diff stain kit), periodic acid-Schiff (PAS, Sigma 395-B), Sudan black B (SBB, Sigma 380-B), Oil red O (Humason, 1979), myeloperoxidase (MPO, Sigma 391-A), acid phosphatase (Sigma 181-A), alkaline phosphatase (Sigma 85L-1), and napthol AS-D chloroacetate
(specific) esterase (Sigma 90-C2) and [alpha]-napthol acetate (non-specific) esterase (Sigma 90-A1).