After three more washes with HRP wash concentrate, the enzyme reaction was developed by adding tetramethylbenzene chromogen
and incubating in the dark for 30 minutes at room temperature.
Aminoethylcarbazole was used as chromogen
, and sections were counterstained with hematoxylin.
This additive is believed to assist the formation of the chromogen
by in 2 ways: (i) the destruction of hydroxylamine produced during the hydrolysis of diacetyl monoxime to diacetyl, and (ii) catalysis of the reaction, allowing less acid to be used (Taylor and Vadgama 1992).
Staining was then visualized by using 3,3'-diaminobenzidine chromogen
8% and specificity of 100% using a single chromogen
for all 3 antibodies.
Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen
This dual chromogen
medium enables the isolation of Salmonellae (including Salmonella typhi and S.
Determination of blood glucose using an oxidase peroxidase system with a non-carcinogenic chromogen
The formaldehyde produced is measured spectrophotometrically with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald) as the chromogen
(Wheeler et al.
, 3-methyl-2benzothiazolinone hydrazone (MBTH, Sigma Aldrich) was added to the substrate.
The interference by reducing compounds such as ascorbic acid primarily consists of either competition with the chromogen
in the peroxidase-catalyzed reaction of hydrogen peroxide or bleaching of the color being formed.
0 ml of working solution [containing one part of chromogen
solution (ferrozine 40 mmol/L and sodium acetate 400 mmol/L) and four parts of buffer/reductant solution (Guanidine chloride 1.