Parameter Experimental Groups Sedentary Body weight (g) 368 [+ or -] 13 Water intake (ml/24h) 15 [+ or -] 5 Citrate synthase
(mol/mg/min) 13 [+ or -] 0.
activity measured in the muscle and the digestive gland of scallops (Placopecten magellanicus) collected during 2003 near Magdalen Islands (Quebec, Canada).
As expected, the voluntary running model enhanced citrate synthase
activity but caused no changes in absolute muscle mass or fiber size.
EGR, exponential growth rate; PK, pyruvate kinase activity; CS, citrate synthase
activity; K, potassium concentration; DRY, fraction dry matter.
Surprisingly, MCI PBMCs showed higher activities of two enzymes regulating the energy machine, cytochrome C oxidase and citrate synthase
, involved in the respiratory chain processes and Krebs cycle, respectively.
Size effects on the activity of citrate synthase
and cytochrome C oxidase were tested using regression (enzyme activity vs.
2]) consumption and citrate synthase
activity in slow- and fast- twitch oxidative and glycolytic skeletal muscle fibers of rats.
DNA sequences from the 21 PCR citrate synthase
A gene amplicons (GenBank accession nos.
Mitochondrial (mt) DNA content, citrate synthase
activity, transcript levels of some nuclear- and mitochondrial-coded genes (cytochrome c oxidase subunit IV [COX-IV], 16S rRNA, COX-I) and of some factors involved in the mitochondrial biogenesis signaling pathway (peroxisome proliferator-activated receptor gamma [PPARgamma] coactivator-1alpha [PGC-1alpha], mitochondrial transcription factor A mitochondrial [TFAM], mitochondrial transcription factor 2B [TFB2]), as well as the protein content of PGC-1alpha were determined.
Notably, other MRC activities, as well as citrate synthase
, a mitochondrial marker enzyme, were largely unaffected.
(CS) and octopine dehydrogenase (ODH) activities were used as indicators of aerobic and anaerobic metabolic potential respectively.
In additional, we confirmed that the concentration of citrate synthase
, a marker of ExT efficacy, in soleus muscle was significantly higher in ExT than in Sed rats.