A survival model with covariates (semi-parametric model) was then used to estimate the effect of the climatic seasons and the local habitats on overall survival.
Using the survival model with co-variables, and firstly considering the climatic seasons as a co-variable, it was found that neither the spring nor the summer was significant (p = 0.
The Cox survival model with co-variables (jointly considering the climatic seasons and the localities) that are specific to each transition (T1, T2, T3, and T4) showed that the transitions from larvae to death (T2) and from pupae to death (T4) are significant in the summer (p = 0.
Thus, the effects of the climatic seasons on T2 and T4 (larvae to death and pupae to death) are higher in the summer than the spring, indicating the decreased risk of the death of larvae and pupae in the summer (negative sign in the product).
Therefore, the main objectives of this study were to determine the development time of the different stages, the relationship between the climatic seasons and local habitats on the time at which the specimens remain in each stage of development and on the transitions to the subsequent stages of metamorphosis or death in Anopheles pseudopunctipennis and Anopheles argyritarsis.
With respect to the co-variables, which represent the climatic seasons and the local habitats, the transitions from larvae to death and from pupae to death were found to be significant in the summer when the risk of larval death decreased, and the same was observed with the pupae.
These results will also raise awareness of the survival time of larvae (48 h) and the relationship between the climatic seasons and localities on the time at which the specimens remain at each stage and the transitions to different stages, i.
Climatic seasons Spring Summer Transition (T) Coefficient p-value Coefficient p-value 1 0.