cloning vector

(redirected from Cloning vectors)
Also found in: Medical.

cloning vector

[′klōn·iŋ ‚vek·tər]
(cell and molecular biology)
A carrier, such as a bacterial plasmid or bacteriophage, used to insert a genetic sequence, such as a deoxyribonucleic acid fragment or a complete gene, into a host cell such that the foreign genetic material is capable of replication.
References in periodicals archive ?
Agilent will also offer a set of catalog components for its leading-edge SureVector cloning kits, enabling the creation of over 1 million different cloning vectors.
The plasmid pUC19 is one of a series of plasmid cloning vectors and it is one of the most widely used vector molecules as the cells into which foreign DNA has been introduced, can be easily distinguished from the non-recombinants based on color differences of colonies on growth media (Storkle and Dominic (2007).
With primers 8F and 1391R which are relevant to bacterial species, the PCR of 16S rDNA was amplified using cloning vectors specifically that of PCR4.
This cloning method allows transferring of DNA fragment into different cloning vectors without using restriction endonucleases and ligase.
Cloning vectors used for transformation was pBI121 for overexpression construct, pGEMT-Easy vector for primary cloning of TYDC and Cor and pTZ57R/T vector for insertion genes of interest in E.
After moving to National Institutes of Health as Visiting Scientist in 1983, he developed a high-efficiency transfection method for delivering cDNA libraries into mammalian cells and cDNA cloning vectors that enable selection on stable cDNA expression.
Most plasmid cloning vectors carry at least one gene that confers antibiotic resistance on the host cell, and selection for transformants is achieved simply by plating on to an agar medium that contains ampicillin antibiotic.
A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.
The claims are directed to a modified glyphosate resistance gene, cloning vectors containing the gene, various plant cells containing the gene, and a process for inserting the gene into cells.
The amplified DNA was then prepared for insertion into cloning vectors by restriction with BarnHI and NdeI.
Scientists have been attempting to construct a variety of food-grade cloning vectors for use in lactic acid bacteria and to improve the bacteriophage resistance of dairy starter cultures using genetic engineering techniques.