immunoprecipitation

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immunoprecipitation

[‚im·yə·nō·prə‚sip·ə′tā·shən]
(immunology)
A protein purification method which involves the formation of an antibody-protein complex to separate out the protein of interest.
References in periodicals archive ?
Control cells (non-transfected) and cotransfected cells were homogenized in M-PER Mammalian Protein Extraction Reagent (Pierce Coimmunoprecipitation Kit, Thermo, Rockford, USA), incubated at room temperature for 5 min, and spun at 13,000xg for 10 min at 4[degrees]C.
A few examples of specific topics include locomotor activity in rodents, automated video image analysis of larval zebrafish locomotor rhythms, identification of clock genes using difference gel electrophoresis, quantitative polymerase chain reaction, coimmunoprecipitation assay, manipulation of mammalian cell lines for circadian studies, and immunocytochemistry on suprachiasmatic nucleus slices.