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In order to observe the accuracy the counts were also made with Coulter counter (Beckman Z2) as well as image analysis based counter (BioRad TC-10) with the same bead suspensions of unknown bead number used for micro-capillary bead counter.
The counting and sizing of spermatozoa from ten animal species using a Coulter counter.
For comparative studies, counts were also performed using the Z2 Coulter Counter instrument according to manufacturer's instructions using the same starting suspension and serially diluted samples.
Coulter Counter Assay: To determine the growth inhibitory activity, MDA-MB-453 human breast cancer cells were exposed to increasing concentrations of black cohosh extracts containing 1%, 15%, or 27% triterpene glycosides (Table 1) or purified triterpene glycosides from Cimicifuga species (Table 2) for 96 h and the number of viable cells was determined by the Coulter Counter assay.
We don't want to just duplicate the capability of a Coulter Counter," he said.
After 3 days, one set of cells was harvested and counted at time 0 with a Z1 model Coulter counter (Coulter Corporation, Miami, FL).
The samples were tested for total solids and turbidity using standard methods and a coulter counter and the results were analyzed.