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Cyanine
(redirected from Cy3 and Cy5)

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Cyanine 

any one of numerous organic compounds containing two heterocyclic radicals connected by a chain consisting of an odd number of methine groups:

where Y and Y’ are O, S, Se, CR2, or some other element or radical, R and R’ are H or van alkyl, X is Cl, Br, I, or some other anion, and n = 0–5.

The general name for this class of compounds is derived from the first compound of the class, bright blue cyanin, or cyanine blue (from the Greek kyanos, “blue”). Depending on the number of methine groups in the chain, a distinction is made between simple cyanines (monomethines), in which n = 0, carbocyanines (trimethines), in which n = 1, dicarbocyanines (pentamethines), in which n = 2, and so forth. The basic method for the synthesis of cyanines involves the condensation of quaternary salts of heterocyclic compounds. Cyanines are polymethine dyes.



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Protein lysates from tumor and normal tissue are labeled with fluorochromes Cy3 and Cy5, respectively, and cohybridized for 1 hour to commercially or self-made antibody arrays.
3 Materials Slides from Full Moon Biosystems (Sunnyvale, CA) (1) with separate blocks of successive dilutions of Cy3 and Cy5 dyes were used including four slides from each of three different lots, with warranty expiration dates of September 2005 (lot C) and July 2006 (lots A and B).
 
 
 
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