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any one of numerous organic compounds containing two heterocyclic radicals connected by a chain consisting of an odd number of methine groups:

where Y and Y’ are O, S, Se, CR2, or some other element or radical, R and R’ are H or van alkyl, X is Cl, Br, I, or some other anion, and n = 0–5.

The general name for this class of compounds is derived from the first compound of the class, bright blue cyanin, or cyanine blue (from the Greek kyanos, “blue”). Depending on the number of methine groups in the chain, a distinction is made between simple cyanines (monomethines), in which n = 0, carbocyanines (trimethines), in which n = 1, dicarbocyanines (pentamethines), in which n = 2, and so forth. The basic method for the synthesis of cyanines involves the condensation of quaternary salts of heterocyclic compounds. Cyanines are polymethine dyes.

References in periodicals archive ?
4, S/N in the Cy3 block was higher than in the Cy5 block, with the measurements of S/N of slide A2 higher than those of the other heavy use slides in the Cy3 block.
Preparation of CyDye DIGE flour stock solution: CyDye DIGE flour, minimal labeling kit, 5 nmol (Cy2, Cy3, Cy5, GE Healthcare Bio-Sciences, Sweden) was left alone 5 minutes at normal temperature in light cutoff by putting out at -20[degrees]C.
Briefly, 120 pg of total protein from each exposed sample and from each control sample was labeled with 400 pmol of the cyanine dyes Cy5 and Cy3 (GE Healthcare), respectively.
Split-control hybridization, The same cDNA control sample was split and labeled separately with either Cy3 or Cy5.
Signal intensities in the Cy3 and Cy5 channels were normalized using total average signal intensity in both channels to generate a balance coefficient.
Additionally, a scatterplot of Cy3 versus Cy5, together with the original image, is available for visual inspection for quality control purposes.
After hybridization, the GEM was scanned at 10-lam resolution to detect Cy3 and Cy5 fluorescence.
We Cy5-labeled a [beta]-chain-specific reporter mAb by reaction with a FluoroLink[TM] Cy5 reactive dye (GE Healthcare), leading to 5 dyes/antibody as measured with dye quantification by ultraviolet/visible spectrophotometry.
The-RNA of five individual Cyp1a2(+/+) wild-type mice and five individual Cyp1a2(-/-) knockout mice were each separately labeled with both Cy3 dUTP and Cy5 dUTP.
After isolation of mRNA using Trizol, mRNA from two sources (for example 4th generation 2,4-D and 4th generation control) was labeled with Cy5 and Cy3 using PCR and hybridized to a Drosophila microarray.
Protein micro-array: Antibodies spotted onto micro-array slides captured specific receptors on the parasite lysates, whereas the detection antibody coupled to the fluor Cy5 bound this complex thus providing a signal that was measured (Fig.