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cytopathic effect

   Also found in: Acronyms, Wikipedia 0.02 sec.
cytopathic effect [¦sīd·ə¦path·ik i‚fekt]
(cell and molecular biology)
A change in the microscopic appearance of cells in a culture after being infected with a virus.


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When cytopathic effect was obvious, DNA was extracted from the supernatant, and the complete hemagglutinin gene was sequenced from a PCR product (forward primer 5'CCATTGGAAAAAACACAGTAC-3', reverse primer 5'CCAAATATATTCCCATAGTC-3'), amplifying a 1,183bp region.
The assay is performed by exposing cell monolayers to stool filtrates, and observing the cells for evidence of cytopathic effect.
The diagnosis of CMV is established by the identification of viral cytopathic effect (intranuclear inclusions) in gastrointestinal mucosal biopsies by routine haematoxylin-eosin (H&E) stains [7].
 
 
 
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