DEAE-cellulose


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DEAE-cellulose

[′dē‚ē′ā‚ē ′sel·yə‚lōs]
(organic chemistry)
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The reagents are used to improve selectivity in the technique analyses or separations, the various reagents include silica gel, DEAE-cellulose, Brij 35, Methyl orange and so on.
Sequential elution of denatured proteins, hydrolyzed RNA, and plasmid DNA of bacterial lysates adsorbed onto stacked DEAE-cellulose membranes.
3]) Crude 3200 384 120 Ion exchane on 1600 256 160 chromatography DEAE-cellulose Affinity 20 51 2550 chromatography on glucose-sepharose
Fractions showing amylase activity were pooled and again applied to (1 X 20 cm) DEAE-Cellulose matrix column (Bangalore genei).
We then pipetted 20 mg DEAE-cellulose resin suspended in 300 [micro]L acetic acid into each well of a 96-well filter plate (Innovative Microplate) and washed each well twice with 0.
in skin care products, such as physical adsorption on chitin, ionic binding onto DEAE-cellulose, covalent bonding on CNBr-Agarose and entrapment in alginate.
The laccase was adsorbed on DEAE-cellulose and Q-Sepharose, but unadsorbed on SP-Sepharose.
The isolation procedure entailed anion exchange chromatography on DEAE-cellulose.
An improvement in electrophoretic transfer of DNA from a gel to DEAE-cellulose membrane.
A homodimeric, fructose-binding lectin was isolated from Del Monte bananas by using a protocol that involved ion-exchange chromatography on DEAE-cellulose and SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75.
4) and desorbed using 1 M NaCl in the starting buffer from the DEAE-cellulose column and SP-Sepharose column.
The isolation procedure entailed ion-exchange chromatography on Q-Sepharose, hydrophobic interaction chromatography on Phenyl-Sepharose, ion-exchange chromatography on DEAE-cellulose, and FPLC-gel filtration on Superdex 75.