DEPC


Also found in: Acronyms, Wikipedia.

DEPC

(organic chemistry)
Mentioned in ?
References in periodicals archive ?
Required mixture was prepared for the reverse transcription reaction in a 20 [micro]l volume as follows: initially, 1 [micro]l template RNA (5 [micro]g) plus 1 [micro]l random hexamer primer (15 pmol) was added into a sterile, nuclease-free tube and reached a 12 [micro]l total volume with DEPC water and incubated at 65[degrees]C for 5 minutes.
The proposed TA impact, outcomes, outputs, and institutional arrangements have been developed in consultation with DEPC, which fully supports the TA.
20 uL reaction volume was used, which contained 10uL of 2X TaqMan gene expression master mixture, 1uL 20X TaqMan gene expression assay, 4uL of cDNA and 5ul of RNase free DEPC treated water was used in a single reaction.
The RNA pellet was washed with 70% ethanol and dissolved in DEPC water.
5 L of PCR master mix (2x) (Fermentas, Germany), 1 L of forward (10 pmoles/ L) and reverse (10 pmoles/ L) primers each (Oligos, GeneLink, USA), and 8 L of DEPC water was subjected to thermocycler (ThermoElectron, Finland) under following conditions; initial denaturation at 94C for 4 min followed by 35 cycles, each of 94C for 30 seconds, annealing at 55C for 1 min and extension at 72C for 1 min.
Total RNA was precipitated in isopropanol washed with ethanol and dissolved in DEPC (diethyl pyrocarbonate) water.
5 [micro]g of oligo-dT primer and DEPC (diethyl pyrocarbonate)-treated water were combined into a microcentrifuge tube to a final volume of 12.
RNA pellet was dissolved in 25 ul of DEPC treated/ RNase free water and stored at - 20degC.
Finally, the dsRNA pellet was washed by 70% ethanol and resuspended by a suitable volume of DEPC treated water.
RNA solution 5ul (approximately 5ug RNA), 2ul of random hexa-nucleotide primers (50ng/ul) and 3ul of DEPC treated water was taken in microfuge tube (Eppendorf, Germany).
1% DEPC water, then packed with sterile and RNase-free silver paper and, after being rapidly frozen in liquid nitrogen, stored at -80[degrees]C for further analysis of SGLT1, VD-dependent calcium-binding protein 28,000 m (CaBP-D28k), PepT1 in the duodenum, and liver fatty acid-binding protein (L-FABP) in the jejunum.