DEPC


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DEPC

(organic chemistry)
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Because the second strands of cDNA did not attach to the plates, the cDNA could be dissociated from the plate by addition of boiling DEPC water.
The Blumberg and Lodish method also advises the addition of DEPC in the solutions to be used in the procedure.
The assessments in phase one and two is the first step in helping DEPC to determine the cost and types of services that would benefit special needs children in their communities.
The integrity of extracted RNA following CTPM, particularly with addition of DEPC, was significantly improved, although 28S and 18S ribosomal bands were degraded in most samples.
The extracted RNA was dissolved in DEPC water, and then stored at -80[degrees]C for further analysis.
The mRNA pellet was dissolved in DEPC treated water and used immediately for construction of cDNA library or stored at -150[degrees]C for other purposes.
1% DEPC (diethylpyrocarbonate) treated water and all work stations and equipment that came into contact with the samples were cleaned with an RNase removing detergent (RNase zapp, Ambion) to eliminate RNA degrading enzymes.
DEPC water contains diethyl pyrocarbonate, which degrades any trace of RNases.
After the ethanol was dried, the RNA pellet was dissolved in DEPC treated water.
1 to 5 pg of RNA was mixed with 1 [micro]l of random hexamer primer or oligo primer and then added to DEPC water until the volume reached 12 [micro]l.
Abbreviations: Bat, Batimastat; BMP, bone morphogenetic protein; CLT, codonolactone; DEPC, diethylpyrocarbonate; EC, endothelial cell; ELISA, enzyme-linked immunosorbent assay; FRET, fluorescence resonance energy transfer; HUVECs, human umbilical vein endothelial cells; MMPs, matrix metalloproteinases; MVD, microvessel density; Runx2, Runt-related transcription factor 2; SFP, L-sulforaphane; TGF-[beta], transforming growth factor beta; VEGF, vascular endothelial growth factor; VEGFR, vascular endothelial growth factor receptor.