melting temperature

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melting temperature

[′melt·iŋ ‚tem·prə·chər]
(biochemistry)
The temperature at which denaturing occurs for half of the double helices of deoxyribonucleic acid.
References in periodicals archive ?
The book's last part includes commented source code for applicatons such as sequence manipulation, filtering vector contamination, calculating DNA melting temperature, parsing a genbank file, and inferring splicing sites.
Betaine, already described as lowering the DNA melting temperature (23), also exhibited this characteristic on both amplicons, but only at concentrations >1 mol/L.
In conclusion, trehalose is a compatible solute that greatly facilitates the PCR of GC-rich templates by reducing the DNA melting temperature and thermostabilizing the Taq polymerase.