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PCR fidelity of Pfu polymerase and other thermostable DNA polymerases.
Again, the reader will recall that DNA polymerase activity will proceed until the end of a template is reached, or the reaction is stopped by outside forces.
In this report we have demonstrated a novel method to effectively combine PCR and cycle sequencing into one reaction using a single DNA polymerase, standard oligonucleotide primers, and standard sequencing reagents.
For example, AZT, one of the earliest effective HIV drugs, battles the AIDS virus by interfering with its DNA polymerase, a protein called reverse transcriptase.
The DNA polymerase now moves down one nucleotide length, to position its active site over the new 3'-OH
DNA Polymerase from Thermococcus litoralis in 1990, and more recently the commercialization of Phusion[sup.
By contrast, the combination of a primer pair containing perfectly matched D1152 mutation-specific forward primer 1152F and reverse primer 1152R, Pfx PCR buffer from the Accuprime Pfx DNA Polymerase Kit (Invitrogen), Accuprime Taq polymerase (without proofreading activity; from the Accuprime Taq DNA Polymerase System), and TagMaster PCR Enhancer (Eppendorf) allowed consistent detection of 5 copies of the CF D1152H mutant allele in the presence of up to --100 000 copies of the wild-type allele without interference from the wild-type sequence.
Previous studies had shown that DNA polymerase tends to select adenine for empty spots.
After initial activation of AmpliTaq Gold DNA polymerase at 95 [degrees]C for 10 min, 40 PCR cycles of 95 [degrees]C for 15 s and 60 [degrees]C for 1 min were performed.
In vivo, DNA polymerase catalyzes the synthesis of the daughter strands of DNA by adding nucleotides that bind to the original DNA strand in complementary base pairs (bp).
The DNA polymerase is a reverse transcriptase, the kind of enzyme that HIV and other retroviruses use to copy their RNA-encoded genetic information into an infected cell's DNA (SN: 5/9/92, p.
Contract award: providing taq dna polymerase with their buffers for in vitro diagnostic use.

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