To understand more completely how DNA supercoils
and the forces that make the strands writhe, Tamar Schlick, a mathematician and Howard Hughes Medical Institute researcher at New York University, and her colleagues have developed a computer model that links knot theory to biochemistry
Comet assay, also known as single cell gel electrophoresis (SCGE), is based on a principle that when the sperm DNA breaks, DNA supercoils
become loose and the negative charges are exposed.
For DNA gyrase, this topoisomerization reaction results in introduction (or removal) of DNA supercoils
, thus affecting the negative supercoiling of DNA necessary to initiate DNA replication and remove positive supercoils that accumulate before an advancing replication fork.