Effect of age on the association between p16CDKN2A methylation and DNMT3B
polymorphism in head and neck carcinoma and patient survival.
Alterations in the expression of genes (MEG3, DNMT1, DNMT3A, DNMT3B
, p53, and HOTAIR) after 48 h exposure to DNC (Fig.
expression might contribute to CpG island methylator phenotype in colorectal cancer.
Therefore, we investigated the association between DNMT3B
genotype and the risk of breast cancer incidence among sporadic breast cancer patients in Fars Province, Southern Iran.
Se han encontrado mutaciones en el gen DNMT3B
en individuos con una rara enfermedad genetica denominada sindrome ICF (inmunodeficiencia, inestabilidad centromerica y anomalias faciales), relacionado con hipometilacion de ADN satelite y descondensacion cromosomica especifica (20).
0001) for RUNX3 gene but not to DNMT3A, DNMT3B
e DNMT3 genes on CpG islands analyzed.
The de novo DNA methytransferases DNMT3A and DNMT3B
, as well as the co-factor DNMT3L, are major players in the general mechanisms underlying DNA methylation, (72-75) but the locus specificity is not explained by this alone.
DNMT3A and DNMT3B
are involved in de novo methylation, whereas DNMT1 maintains hemimethylated DNA during replication.
DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome.
The methylation of CpG sites within the human genome is catalyzed by four well-documented DNA methyltransferases (DNMTs) named DNMT1, DNMT2, DNMT3A and DNMT3B
86) DNMT3A-R882H mutant lacks the DNA methyl transferase activity but still binds the wild-type DNMT3A and DNMT3B
, suggesting that R882H is a dominant-negative mutant.
Two hundred seventy-seven SNPs (single-nucleotide polymorphisms) within the range of 20 kb up- or downstream of the following genes were genotyped: DNMT1, DNMT3A, DNMT3B
, DNMT3L, TET1, TET2, TET3, TDG using the Illumina HumanHap550, HumanHap550-Duo or Human610-Quad BeadChip microarrays as described previously (Torgerson et al.