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DNase |
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DNase (biochemistry) How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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001 [micro]g of RNase A (Qiagen, Hilden, Germany) and 1 [micro]L (2 U) of Turbo DNA-free DNase I (Ambion, Austin, TX, USA) with 1x Turbo DNA-free buffer were incubated at 37[degrees]C for 30 min under conditions that prevented destruction of viral RNA in the viral particles. The recRNA was subsequently treated with RNase-free DNase (Boehringer Mannheim, Mannhein, Germany) to remove DNA template, recRNA was quantified by measuring its absorbance at 260 nm. Disease-producing bacteria seem to make more of an enzyme called DNase than benign microbes do, Nizet says. |
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