Recombinant human DNase I has been developed clinically for treatment of pulmonary disease in patients with cystic fibrosis .
2001) DNase I mediates internucleosomal DNA degradation in human cells undergoing drug-induced apoptosis.
DNase I and fragmented chromatin during nuclear degradation in adult bovine lens fibersMol.
The activity of alkaline DNase is the result of a group of enzymes which depolymerise DNA in alkaline situations, whereas that of acid DNase, a group of enzymes have optimal activity in acid pH.
A DNase I high concentrate stock solution was produced at 40 mg/mL using DNase I and nuclease-free water.
A DNase kit was then used to test all of the DNase I standards, following the same protocol as with the RNase.
When the number of viral particles in the sample was high, we omitted the RNase A and DNase I
treatments and used the RNeasy Mini Kit (Qiagen) for RNA extraction.
Human DNase I was purified from urine as described previously (8).
An assay reagent set for DNase I activity was prepared in two steps: production of a gel plate for keeping the CAM wet, and preparation of CAM containing reaction buffer.
To measure whether ample DNase I can clear the decks and prevent such an onslaught, the researchers created mice that lack the enzyme and compared them with healthy mice.
Moroy and his colleagues examined 69 mice missing the gene that encodes DNase I, as well as 78 others that had a partial enzyme deficiency and 37 that had a full complement of the enzyme.
is a naturally occurring body enzyme that chops up DNA, a major constituent of the sticky phlegm that accumulates in the lungs and other organs of cystic fibrosis patients.