depurination


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depurination

[dē‚pyu̇r·ə′nā·shən]
(biochemistry)
Detachment of guanine from sugar in a deoxyribonucleic acid molecule.
References in periodicals archive ?
It can act by causing acid catalyzed depurination, randomly removing A and G bases from DNA and leaving "gaps" or chain breaks which can't be amplified across.
Others discuss DNA self-catalyzed depurination, B12-dependent photoreceptors, engineering and in vivo applications of riboswitches, cyclic GMP-AMP as an endogenous second messenger, antibiotics, surface-active and surfactant proteins, functionally diverse lipid-binding compartments, the evolution of membrane coating complexes, endoplasmic reticulum-plasma membrane contact sites, mitochondrial biogenesis, oxidative stress, mammalian peroxiredoxins, redox-based regulation of bacterial behavior, heme uptake and bacterial cell membranes, biological probes built from fluoresceins and rhodamines, microbial rhodopsins, and cellular electron cryotomography.
The depurination rate of single-stranded DNA is 4 times higher than that of double-stranded DNA (33).
Evidence that a burst of DNA depurination in SENCAR mouse skin induces error-prone repair and forms mutations in the H-ras gene.
Heating in the presence of a strong acid causes depurination (the release of the purine bases) and hydrolysis of the RNA strands to give free pyrimidine nucleotides, ribose, and phosphate.
After electrophoresis, the DNA was partially hydrolyzed (15 min depurination in 0.
25 N HC1 for 10 minutes to allow partial depurination and cleavage of large fragments, in denaturation solution composed of 0.
If the environment is more acidic (low pH), acid depurination is promoted, and if it's more alkaline, hydrolysis of the sugar-phosphate backbone increases.
2] can interact with DNA and, in turn, lead to depurination of DNA--a potential mutagenesis event (Cavalieri et al.
This depurination of the SR loop results in irreversible inhibition of protein synthesis at the translation step by impairing both the elongation factor (EF) 1-dependent binding of aminoacyl-tRNA and the GTP-dependent binding of EF-2 to the affected ribosome (Gessner and Irvin, 1980).