Digitonin


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digitonin

[‚dij·ə′tō·nən]
(pharmacology)
C41H64O13 A glycoside derived from the purple foxglove plant (Digitalis purpurea); a white powder melting at 255-256°C; used as a medicine for cardiac conditions.

Digitonin

 

a steroid saponin with surface-active properties; it is used to separate enzymes. It causes mitochondria to decompose into protein complexes that participate in conjugated enzyme processes not observed in mixtures of isolated enzymes.

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3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS) and 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulphonate (CHAPSO) were from Calbiochem (Darmstadt, Germany); N-(2-hydroxyethyl)-piperazine-N'-(2-ethanesulphonic acid) (Hepes), tris(hydroxymethyl)aminomethane (Tris), ethylene glycol-bis([beta]-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), 1,6-diphenyl-1,3,5-hexatriene (DPH), sodium dodecyl sulphate (SDS), phenylmethylsulphonyl fluoride (PMSF), aprotinin, benzamidine, digitonin, cholic acid, deoxycholic acid, n-dodecyl-[beta]-D-maltoside (DBM), and dodecylpoly[(ethylene-glycolether).
2+] to totally quench the Fura-2 fluorescence after the addition of digitonin (50 taM), according to the method described by Sage (19).
14]C]-radio-labeled VLCFA substrates and one of two available methods: either in intact human skin fibroblasts cultured in monolayer (5); or in isolated fibroblasts permeabilized with digitonin (6).
The first colorimetric assay for cholesterol in serum, reported by Grigaut in 1910, combined purification by digitonin with an acid reagent.
Combination of the saponin digitonin (5 [micro]M), which also inhibits P-gp, with SM significantly enhanced the inhibition of P-gp activity.
The comparative method was based on the use of 1 g/L digitonin for 35 s before centrifugation and resuspension of the pellet in MOPS buffer (12).
Briefly, membrane and cytosolic fractions were preincubated with the salt solution for 10 min at 37 [degrees]C and incubated for another 15 min in the presence or absence of 100 [micro]M PKC-specific peptide substrate, subsequently with 50 [micro]g/m1 digitonin and 100 [micro]M ATP mixed with [[gamma]-[.
Digitonin in phosphate-buffered saline (PBS), at final concentrations of 0, 20, 45, and 90 [micro]mol/L, was used to control the degree of whole-blood hemolysis (21).
Treatment with 40[micro]M digitonin increased the number of parasites marked with PI by 68.