Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA
The small conditional RNAs trick cancer cells into self-destructing by selectively forming long double-stranded RNA
polymers that mimic viral RNA.
Dicer is a critical enzyme involved in the RNAi gene silencing cascade and acts as the natural initiation point for this pathway by processing double-stranded RNA
so that it can be used for gene silencing.
They found that the RIG-I protein moves back and forth (translocates) selectively on double-stranded RNA
, and that this activity is greatly stimulated in the presence of 5'-triphosphate.
20050244858 Methods and Compositions for the Specific Inhibition of Gene Expression by Double-Stranded RNA
-- 20050277610 Methods and Compositions for the Specific Inhibition of Gene Expression by Double-Stranded RNA
About Nastech's RNA-Based Therapeutics Programs
Ten double-stranded RNA
genome segments were separated by electrophoresis, showing a pattern typical of ARV with the S1 segment migrating between S- and M-segment classes (5).
The RNAi gene silencing process involves the introduction of double-stranded RNA
molecules into a cell, after which a multistep cellular process creates single-stranded siRNA moleculesthat interfere with the translation of mRNA into the protein it encodes.
Working in the mid-1990s with a roundworm, Fire and Mello found that double-stranded RNA
with a given sequence shuts down protein production of the gene with a matching sequence.
In the late 1980s, geneticist Richard Jorgensen, then working at a California plant biotechnology company, attempted to deepen the hue of purple petunias by introducing more of the gene that gives them their color, in the form of double-stranded RNA
Segmented double-stranded RNA
viruses; structure and molecular biology.
activates biochemical machinery which degrades those mRNA molecules that carry a genetic code identical to that of the double-stranded RNA
Herpes simplex virus 1 (HSV-1), which causes cold sores, uses a short, double-stranded RNA
to outwit a cell's defensive measures.