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For amplification of RET exon 21 and the EDNRB and GDNF genes, we generated new pairs of primers (Table 1).
The RET and the EDNRB signaling pathways are critical for the normal development of the ENS (18, 20, 21).
P383 L386delinsP, D241D (c723T>C), N426N (c1278T>C), and IVS4 -14T>C] in the EDNRB gene.
Mutations in EDNRB and EDN3 were scarce and were mainly inherited from unaffected parents.