Epithelial membrane antigen
– A diagnostic discriminant in surgical pathology: Immunohistochemical profile in epithelial, mesenchymal, and hematopoietic neoplasms using paraffin sections and monoclonal antibodies.
Immunohistochemical markers showed positivity for calretinin and epithelial membrane antigen
, and negativity for CD34, CD117, smooth muscle actin, desmin, and S100.
Abbreviations: LPM- Lymphoplasmacyte- rich meningioma; WHO- World Health Organisation; IHP- idiopathic hypertrophic pachymeningitis; EMA- Epithelial membrane antigen
Immunohistochemistry of MPMNs demonstrates positivity for CD56, progesterone receptor, epithelial membrane antigen
, and vimentin, which is similar to PPMs.
Immunohistochemical staining (diaminobenzidine x20) showed cells positive for epithelial membrane antigen
In renal cell carcinoma, hepatocellular carcinoma and metastatic adenocarcinoma, epithelial membrane antigen
(EMA) is positive but synaptophysin is negative, while in ACC, EMA is negative.
Ber EP4 and epithelial membrane antigen
aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin.
The tumor failed to immunostain with antibodies for cytokeratin (CK)7, desmin, epithelial membrane antigen
(EMA), Melan-A, S-100 protein, HMB-45, smooth muscle actin, caldesmon, CD34, CD31, Factor VIII, CK20, CKs AE1/AE3, CK 34betaE12, CAM 5.
The purpose of this study was to see whether this stain was really 100% positive in BCC in our population, to gain confidence in the use of this immunostain and to encourage the fellow histopathologists to use this stain in difficult cases of epithelial tumors especially in combination with epithelial membrane antigen
Immunohistochemical studies--including those with keratin, epithelial membrane antigen
, CK5/6, and p63--are usually performed to confirm the epithelial nature of the spindled tumor, but these markers are absent in as many as 30% of cases.
The mediastinal tumor samples taken from our patient stained positively for vimentin, muscle-specific actin, and desmin and were negative for cytokeratin AE 1/3, cytokeratin 7, cytokeratin 20, epithelial membrane antigen
(EMA), calretinin, S100 protein, melanoma antibody (HMB45), and leukocyte common antigen.
Results were negative for carcinoembrionary antigen and the epithelial glycoprotein Ber-EP4, whereas results were positive for the mesothelial markers cytokeratins, calretinin, epithelial membrane antigen
, and HBME-1, thus leading to the correct diagnosis of peritoneal epithelial mesothelioma.