We suggest that these four MAbs recognize epitope (s) containing the differentiating amino acids located in CH2 and/ or CH3 domains common to IgGl, 2 and 4 subclasses, but not CH1 domain, because our results showed no reactivity of these MAbs with Fab fragments
of IgG 1 molecules (Figures 3 and 5).
Although site-specific bioconjugation was made possible through recombinant Fab fragment
production, the Fab fragments
were not labeled through the thiol groups in the current study because one chelate per Fab fragment
did not yield satisfactory signals.
After the wells were washed four times, 25 [micro]L of the calibrators (PSA calibrators from the ProStatus PSA F/T kit) or serum samples in duplicate and 100 ng of each tracer Fab fragment
in 100 [micro]L of ProStatus PSA Assay Buffer were added.
According to the manufacturer, Digibind is a polyclonal mixture of antibody Fab fragments
having a range of affinities, with dissociation constants (K.
Digoxin specific Fab fragments
in the treatment of oleander toxicity in a canine model.
All these MAbs reacted only with Fc, but not Fab fragments
of their immunogens (Figure 3).
were site-specifically biotinylated with 95 [micro]mol/L maleimide-[PEO.
Brief report: treatment of severe colchicine overdose with colchicine-specific Fab fragments
A 1:1500 dilution of antidigoxygenin-AP Fab fragments
(Roche) was then added, and the embryos were incubated overnight at 4[degrees]C and the reaction was allowed to proceed according to the kit manufacturer's instructions.
The Dower patent covers methods to display Fab fragments
using phage display technology and MorphoSys received a license for the Dower patent from Biosite in January 2000.