We have developed an ion chromatography (IC) assay using ultraviolet (UV) detection following post-column derivatization with ferric nitrate
to determine phytate [inositol hexakis phosphate (iP6)] (1) (2) in wild rice samples and other diet composites.
Solution 1 (Maleic acid) Maleic acid 25 g Distilled water 1000 mL Solution 2 (Redox) Ferric nitrate
30 g Ferrous ammonium sulfate 80 g Citric acid 20 g Distilled water 1000 mL Solution 3 (Detergent) n-Dodecylamine acetate 3 g Synperonic-N 4 g Distilled water 1000 mL Solution 4 (Silver nitrate) Silver nitrate 200 g Distilled water 1000 mL
2]O) volume: 15 [micro]L; ferric nitrate
volume: 100 [micro]L; incubation time: 10 s; first reading: 10 s; number of readings: 6; interval: 10 s; calculation: endpoint; first: M1; last: 6; calibration: each run).
Taft found that when one of these "iron-oxo" complexes reacts with ferric nitrate
in a methanos solution, a green-brown color appears and then gives way to a yellow color when ether diffuses into the system.