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fluorescent in situ hybridization |
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fluorescent in situ hybridization [flə¦res·ənt in¦sit‚chü ‚hī·brə·də′zā·shən] (genetics) A technique in which a deoxyribonucleic acid (DNA) probe is labeled with a fluorescent dye (that can be visualized under a fluorescent microscope) and then hybridized with target DNA, usually chromosome preparations on a microscopic slide. It is used to precisely map genes to a specific region of a chromosome in prepared karyotype, or can enumerate chromosomes, or can detect chromosomal deletions, translocations, or gene amplifications in cancer cells. Abbreviated FISH. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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We performed multicolor fluorescence in situ hybridization on samples from nine men obtained before and during the pesticide spraying season to assess sperm aneuploidy. Numerous companies are working on molecular techniques such as DNA microarrays, polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), and mass spectrometry (MS) to evaluate maternal blood and urine samples. The scope of cytogenetics includes several technologies besides fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and multicolor FISH. |
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