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1996), incomplete Freund's adjuvant (IFA), Freund's complete adjuvant (FCA) (Difco labs, Detroit, MI, USA) and with Alum adjuvant in to the shaved flank and ear of experimental goats.
AcmA, as a vaccine, in combination with both alum and freund's adjuvants could strongly induce in immune-deficient IFN-g secretion and polarize the Th1 pattern.
1 mL complete Freund's adjuvant (CFA) (Sigma-Aldrich) into the right hindpaw as in previous studies (Anagnostou 2009, Tigka 2005).
Freund's adjuvant and its incomplete form: Another conventional adjuvants include complete Freund's adjuvant (CFA) and incomplete Freund's adjuvant (IFA).
Briefly, 100 [micro]g (200 [micro]L) of hK13 was diluted 1:1 in complete Freund's adjuvant for the first injection and in incomplete Freund's adjuvant for subsequent injections and administered subcutaneously three times at 3-weeks intervals.
1 mL of complete Freund's adjuvant is injected into the subplantar region of the right hind paw of the rats.
It consists of a suspension of killed HIV-1 virus particles that have been emulsified with Incomplete Freund's Adjuvant (IFA, a mixture of mannide mono-oleate and a highly purified mineral oil).
The purified fusion proteins that were homogenized in complete Freund's adjuvant were used to immunize male New Zealand rabbits for three times at two-week intervals.
Complete Freund's adjuvant (CFA) is another powerful adjuvant consisting of inactivated Bacillus Calmette-Guerin (BCG) and a mixture of different TLR ligands in a mineral oil solution Although BCG is a strong activator of DCs and induces a Thi response, CFA is not approved for human use(1).
Plasmids were injected intramuscularly, while VLP and Freund's adjuvant (Gibcol, BRL) were injected subcutaneously.
with an emulsion form of Candida albicans surface mannan extract (SM) mixed with either Incomplete Freund's Adjuvant [SM/IFA], or Complete Freund's Adjuvant [SM/CFA] or GA mixed with IFA [SM/GA/IFA].