The new microarray doubles Agilent's number of gene probes
on a single array, while delivering the proven sensitivity, reproducibility and quality of Agilent's open platform.
Cloning and sequencing the 18S rDNA and rbcL gene probes
In general, the rpoB gene amplicons were incubated with immobilized, membrane-bound rpoB gene probes
, including overlapping wildtype sequences (S1-S5) and 4 of the most frequent mutations (R2:Asp516Val, R4a:His526Tyr, R4b:His526Asp, and R5:Ser531Leu).
On gestational day 16, the researchers examined the brain of one fetus from each litter using gene probes
and in situ hybridization to obtain a quantitative measurement of gene activity.
The number of gene probes
has been increased from 14,000 to more than 21,000 genes, which means that the Arabidopsis genes recognized as biologically important are now contained in a single, 60-mer oligonucleotide formatted, microarray.
The new report, titled "Reevaluation of Microbial Water Quality: Powerful New Tools for Detection and Risk Assessment," outlines gene probes
, genotyping, antibody, and polymerase chain reaction (PCR) techniques that promise to replace outdated methods.
Raman techniques under development include multidimensional Raman for the study of molecular dynamics, surface-enhanced Raman gene probes
, and the development of standard Raman spectral libraries.
An in situ hybridization assay, with gene probes
specific for detection of TSV, was negative for TSV in challenged cells.
With gene probes
and monoclonal antibodies introduced at the meeting, the process would take only a few hours.
The additional advantages of the RT-PCR approach are that this method can be readily applied to both serum and tissue samples and that the sequence of the PCR-amplified segment allows conclusions about the relatedness of the detected virus to existing viruses and the synthesis of specific gene probes
for this virus.
Use of gene probes
and adhesion tests to characterise Escherichia coli belonging to enteropathogenic serogroups isolated in the United Kingdom.
Identification of enterotoxin Escherichia coli by colony hybridization using three enterotoxin gene probes