Use of Giemsa
stain to detect changes in acrosome of frozen ram spermatozoa.
Three drops of this solution were placed on slides and stained with 10% Giemsa
solution in Sorensen's phosphate buffer (pH 8.
The slides were then washed in distilled water, air-dried, and stained with Giemsa
(1% solution, diluted in phosphate buffer at pH 6.
After fixing, the slides were stained in aqueous Giemsa
(5%) (Sigma) for 10 min and May-grunwald Giemsa
(Sigma) for 2 min .
Once in the laboratory, we fixed smears in 100% methanol and stained them in a Giemsa
Nova combinacao dos componentes de May-Grunwald e do Giemsa
num so corante de emprego rapido.
Turk's solution preparation: Dilute 3 mL glacial acetic acid to 100 mL total volume with deionized water and add two to three drops methylene blue or Giemsa
ve Toluidin blue ile yapilan boyamada metakromatik sitoplazmik granullerin tespit edildigi kaydedildi.
The slide was dried on a metal heating plate at 35-40[degrees] C and most of them were stained with a 3% Giemsa
solution for 13-15 min.
BAL fluid was centrifuged at 1,200 rpm for 10 min, and the sediment was smeared onto clean glass slides, air-dried, and stained with May-Grunwald-Giemsa
(May and Grunwald 1909; Giemsa
1922-1923) and Perl's Prussian blue (Pearse 1980) for iron deposits.
The presence of highly motile trypanosomes and Giemsa
stained preparations of intestinal contents were identified as T.
the Classic Cytogenetic era of Giemsa
stained whole cell karyotyping, 2.