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beta]-Hexosaminidases A and B are involved not only in the removal of the [beta]-N-acetylgalactosamine residues from the nonreducing terminal of the oligosaccharide of GM2 ganglioside, but also in the removal of GlcNAc in glycoprotein degradation (26-28).
3] Nonstandard abbreviations: LSD, lysosomal storage disease; FOS, free oligosaccharides; MS, mass spectrometry; AGU, aspartylglucosaminuria; ANTS, 2-aminonaphthalene trisulfone; GlcNAc, N-acetylglucosamine; MALDI-TOF/TOF, MALDI-time-of-flight/time-of-flight; DHB, 2,5-dihydroxybenzoic acid; TFA, trifluoroacetic acid; Asn, asparagine; ML, mucolipidosis; GNPT, GlcNAc phosphotransferase.
Percentages of postlarvae on OSE papers in the presence of GlcNAc are shown in Figure 5A.
4]-M concentrations of GlcNAc for 2 h are shown in Figure 5B.
The researchers used chemical tools and molecular modeling techniques developed in their laboratory to determine that GlcNAc inhibits a step in glycolysis (not to be confused with glycosylation), a metabolic pathway that involves 10 enzyme-driven steps.
What's unique here is that the addition of GlcNAc is dynamic and reversible," says Hsieh-Wilson.
Specific binding of Cu(II) to GlcUA at acidic pH and GlcNAc at neutral/alkaline pH has been observed in HA [4, 5].
Whole-plasma profiling showed a much wider spectrum of N-glycans, including fucosylated N-glycans and glycans with a bisecting GlcNAc or truncated third antenna.
A frequently occurring O-linked glycan is the single GlcNAc linked to nuclear and cytosolic proteins.
686 Da detected in the tryptic digests of asialotransferrin spots is consistent with a structure containing 2 GlcNAc, 3 Man, 3 GlcNAc, and 1 Fuc connected to [Asn.
In addition, spectacular differences in the activities of two O-glycosyltransferases, core 2 GlcNAc transferase and a-2,6-sialyltransferase, are related to the abnormal O-glycan synthesis (50, 51).
2]O , distilled, deionized water; and GlcNAc, N-acetylglucosamine.
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- GLCM Activation & Maintenance Area
- GlcNAc phosphotransferase
- GlcNAc-1-phosphotransferase subunit gamma
- GlcNAc-1-phosphotransferase subunits alpha/beta
- GlcNAc-PI synthesis protein
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