They found that another type of enzyme, a-1,4-a-1,4 glucosyltransferases (amylomaltase: Amase), is able to change the side-chain distribution of amylopectin.
In order to obtain thermostable enzymes, a number of thermophilic organisms were screened for the presence of glucosyltransferases.
The cDNA inserts that were used as probes were those homologous to rRNA, and to mRNAs encoding acetyl CoA carboxylase (ACC), lipoxygenase (LO), glucosyltransferase, inorganic phosphate translocator, nitrate reductase, and glycinamide ribonucleotide (GAR) synthetase/GAR transformylase.
Molecular biology of GDH-based biotechnology: Some of the GDH-synthesized RNAs that were used as probes were those homologous to mRNAs encoding glucosyltransferase, phosphate translocator ; acetyl CoA carboxylase (ACC), lipoxygenase (LO) ; probes #1 for nitrate reductase (NR) and probe #2 for glycinamide ribonucleotide transformylase/glycinamide ribonucleotide synthetases (GART/GARS) (Table 1).
The homologous cDNA probe for the mRNA encoding glucosyltransferase was hybridized to the arrays of peanut's GDH-synthesized RNAs but hybridization bands were obtained only with the RNAs synthesized by the GDH of NH4Cl-treated and the Pi-treated peanuts (Fig 6); the GDH-synthesized RNA of control peanut gave no bands.
Optimized dry matter yield: The activity of the GDH-synthesized RNA (probe) that is homologous to the mRNA encoding glucosyltransferase is demonstrated by the differential distribution of the Northern hybridization bands in the arrays of the GDH-synthesized RNAs of the NH4Cl, and Pi-treated peanuts (Fig 6).
4]Cl) minimized the GDH-synthesized RNAs homologous to mRNA encoding glucosyltransferase thus increasing dry matter 11%.