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gram stain
(redirected from Gram staining)

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gram stain

Staining technique for the initial identification of bacteria, devised in 1884 by the Danish physician Hans Christian Gram (1853–1938). The stain reveals basic differences in the biochemical and structural properties of a living cell. A slide containing a smear of bacteria is treated with a purple dye; the slide is then dipped in an iodine solution, followed by an organic solvent (such as alcohol) that can dissolve the dye. Gram-positive bacteria remain purple because they have a thick cell wall that the solvent cannot easily penetrate; gram-negative bacteria lose their colour because they have thin cell walls that allow the solvent to penetrate and remove the dye.



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An example offered in the EIR was in regard to gram staining.
Five observations on the 483 hit the soft water and equipment cleaning issues, citing: * Failure to "speciate" microorganisms to monitor bioburden flora trends in purification areas and conduct gram staining routinely when the firm exceeded environmental limits.
I know that EDTA and other additives can be toxic to bacterial specimens placed in this type of container and are, therefore, not suitable for culturing; however, I do not know of a reference that indicates that it is not suited for holding specimens for Gram staining.
 
 
 
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