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gram stain |
Also found in: Medical, Wikipedia | 0.07 sec. |
gram stainStaining technique for the initial identification of bacteria, devised in 1884 by the Danish physician Hans Christian Gram (1853–1938). The stain reveals basic differences in the biochemical and structural properties of a living cell. A slide containing a smear of bacteria is treated with a purple dye; the slide is then dipped in an iodine solution, followed by an organic solvent (such as alcohol) that can dissolve the dye. Gram-positive bacteria remain purple because they have a thick cell wall that the solvent cannot easily penetrate; gram-negative bacteria lose their colour because they have thin cell walls that allow the solvent to penetrate and remove the dye. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| If signs of culture appeared, a blood sample was taken from the culture bottle and Gram staining on Columbia blood agar (BioMerieux, Marcy L'Etoile, France) and chocolate agar (BioMerieux) was performed. In contrast to other approaches, the MALDI BioTyper does not require any initial assessment like gram staining, oxidase test of unknown samples, choice of PCR primers or usage of selective growth media. Gram staining of the sample showed numerous polymorphonuclear leukocytes and gram-negative bacteria. |
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