Gram's Method

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Gram’s Method


a method for the differential staining of bacteria, proposed in 1884 by the Danish physician H. C. Gram.

In Gram’s method the bacteria are stained with basic dyes, including crystal or methyl violet, which are then fixed in an iodine solution. After rinsing the dyed preparation in alcohol some types of bacteria remain thoroughly stained (the so-called gram-positive bacteria), and others (gram-negative) are decolorized. Such a division is of great importance in the classification of bacteria and in the microbiological diagnosis of infectious diseases.

References in periodicals archive ?
Gram staining was positive in 78% cases and the commonest organisms were gram positive cocci.
Published by Hans Christian Gram in 1884, gram staining distinguishes gram-positive bacteria (purple) from gram-negative bacteria (pink).
Multi-slide cuvette stainers with electrooptical decolorization now offer the benefits of random access gram staining to high-volume microbiology laboratories.
1), including Gram staining, subcultivation, and biochemical identification, we assumed a referenced mean TAT of 48 h (16).
vaginalis, but for the full characterization of the smears the joint application of PCR and Gram staining is the best choice.
Application of this staining to detect Wolbachia was used during the study period and was found to be more efficient than the Gram staining (Fig.
For the time being, Gram staining and bacterial culture remain the cornerstones of bacterial meningitides diagnosis in a clinical setting.
Gram staining was done to differentiate bacteria largely into two groups, Gram-positive and Gram-negative.
Processing of samples: In the bacteriology laboratory, all CSF samples were subjected to direct smear and Gram staining followed by culture on 5 per cent sheep blood agar and chocolate agar (Himedia, Mumbai, India).
When he spots a patient with early signs of wound infection, he makes liberal use of ultrasound to determine if there is a loculated area of fluid collected in the wound, and to guide aspiration of a fluid sample for Gram staining and culture.
After two of these episodes, the hospital began pretransfusion Gram staining and culturing of all platelets but did not delay platelet transfusion awaiting Gram stain or culture results.