Griess reagent

Griess reagent

[′grēs rē‚ā·jənt]
(analytical chemistry)
A reagent used to test for nitrous acid; it is a solution of sulfanilic acid, α-naphthylamine and acetic acid in water.
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An aliquot of the solution of each sample was removed and diluted with Griess reagent comprising of 1% sulfanilamide in 5% H3PO4, and 0.
Nitric oxide production was determined by using Griess reagent kit (Molecular Probes Life technologies).
Griess reagent kit, Dulbecco's modified eagle medium (DMEM), and fetal calf serum (FCS) were purchased from GIBCO/Life Technologies Inc.
Contract awarded for Griess reagent (modified) 2ea and other 19 species
5 ml of Griess reagent was added to one drug-free control tube to observe any color change (strong or weak pink).
5 mL of Griess reagent was added to one drug free control tube to observe any colour change (Strong or weak pink).
Freund's adjuvant complete (CFA), N-methoxysuccinyl-Ala-AlaPro-Val p-nitroanilide and Griess Reagent system were purchased from Sigma Chemical Co.
Griess reagent was prepared by solving 1g sulfanilamide in 100 ml phosphoric acid 5% mixed with 0.
The Griess reagent is based on the two-step diazotization reaction in which acidified nitrite produces a nitrosating agent, which reacts with sulphanilic acid to produce the diazonium ion.
As the blank distilled water, to which Griess reagent was added, is used.
The Griess reagent consists of sulfanilamide (58 mM in 3 M HCl) and N-1-naphtylethylenediamine (722 [micro]M).
Nitric oxide scavenging activity was performed by Griess reagent method.