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Ion-exchange HPLC has the added advantage of being able to identify the presence of variants such as S, C, D, and E in their heterozygous state, as well as fetal Hb, CHb, and LA1c.
HPLC columns: Stainless-steel or plastic columns packed with beads gels (spherical particles).
Pictured, front from left, are David Walton and Lynda Bending from HPLC, with Adam Rock, Matt Kitson, David Pugh and Ian Pitt from Bruton Knowles and Julie Boardman from HPLC
Serotonin in body fluids: characterization of human plasmatic and cerebrospinal fluid pools by means of a new HPLC method.
5 and 25 [micro]g/ ml for d4T) that were prepared in water and run along with the unknown solutions with each run by HPLC and spectrophotometric methods.
The HPLC system consisted of a quaternary gradient pump unit, a column oven, and a diode array spectrophotometric detector (DAD, all Series 200 instruments from Perkin Elmer, Norwalk, CT, USA), a manual injector from Rheodyne (Rohnert Park, CA, USA), and a Zorbax C8 4.
The teens in the HPLC group were placed on a diet limiting them to 20 g/day of carbohydrates.
Because smaller particles produce significant hack pressures, conventional HPLC systems are prone to leaks and system failures.
All solvents were HPLC grade from Burdick and Jackson (Muskegon, MI).
If you read this column you know we (and most analytical chemists) favor HPLC analyses over all others where it is applicable.
The HPLC needs as little as a hundredth of a drop for analysis.
Waters Corporation (Cambridge, MA; 617-876-9300) will host a live, interactive audio press briefing from HPLC '99 in Granada, Spain, on June 2, 1999, to announce the launch of the company's newest HPLC column brand.
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