Hematoxylin

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hematoxylin

[‚hē·mə′täk·sə·lən]
(organic chemistry)
C16H14O6 A colorless, crystalline compound occurring in hematoxylon; upon oxidation, it is converted to hematein which forms deeply colored lakes with various metals; used as a stain in microscopy.

Hematoxylin

 

a dye used in microscopy for staining plant and animal tissues. Hematoxylin is extracted by ether from the colored wood of the logwood tree, which is native to Central America and the Antilles Islands. In the course of its preparation for use in microscopy, the substance is “matured,” or oxidized to hematein, which stains cell nuclei, chromosomes, and cell membranes a blue or blue-black color.

REFERENCE

Romeis, B. Mikroskopicheskaia tekhnika. Moscow, 1953. (Translated from German.)
References in periodicals archive ?
The sections were counter stained with Mayer's haematoxylin and mounted with DPX mountant.
Haematoxylin and eosin stained four-micron sections showed a well circumscribed tumor composed of hyperplastic acini retaining the configuration of normal breast lobules (Fig.
Different spectrophotometric procedures have been reported for determination of PEH including the formation of ion-pair complexes between the drug and alizarine, alizarine red S, alizarine yellow G or quinalizarine [14], ninhydrin in sulfuric acid [15], nitrobenzene derivates in acetonitrile medium [16], oxidative coupling with 4-aminoantipyrine in the presence of potassium ferricyanide or sodium periodate [17,18], diazotized p-nitroaniline or 2-aminobenzothiazol in alkaline medium [19,20], forming a charge transfer complex with chloranil or haematoxylin in alkaline medium [21,22]= 7and[23].
Every 3 serial sections of duodenum portion of intestine were designated as a group, and within each group, the first section was stained with Haematoxylin and eosin (HE) to display epithelia cells; the second one was stained with antisense probe of Cdc25B mRNA, and the last one was used as control of ISHH, in which the antisense probe was replaced with sense probe of CDC25B mRNA.
The 40-[micro]m-thick sections were stained with Mayer's haematoxylin and 0.
Characteristics at Haematoxylin and Eosin staining of ruptures of the long head of the biceps tendon.
It was concluded that the tumour represented an adrenal cortical carcinoma as it had the correct haematoxylin and eosin appearance for an adrenal cortical tumour.
The specimens then were saturated in liquid paraffin for 24 hours and stained with haematoxylin and eosin.
Haematoxylin and eosin (H&E) staining was used for showing general cell morphology in the spontaneous differentiation experiments.
The histology of the thymus after proper processing and staining with haematoxylin and eosin, when compared with the pre-transplant same thymic tissue, showed growth and proliferation of the thymic tissue with Hassall's corpuscles, scattered and aggregated lymphocytes showed comet like structures.
Transverse sections of 5 [mu]m thickness were cut at 200 [mu]m intervals and three sections of each artery were stained with haematoxylin and eosin and three with elastic van Gieson.
FNAC cytology material was spread directly on to glass slides and fixed in isopropyl alcohol and smears were stained with haematoxylin and eosin.