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For all the samples, the total WBCs and differential cell counts were performed, first manually by a haemocytometer and secondly via automated analysis.
1 mI of liquid culture was examined with Haemocytometer under light microscope and culculated spores.
Leukocytes (WBCs) count 103 /mm3 was calculated by Haemocytometer method (Kolmer et al.
The erythrocyte and leukocyte counts were determined by dilution and counting in a haemocytometer.
2) Yeast cell count was carried out using a haemocytometer, and all statistical analysis was carried out using XLSTAT 2014.
Cells density and viability were assessed by the trypan blue dye exclusion method using a haemocytometer (Neubauer improved, Brand).
Intial percent motility of the spermatozoa was assessed microscopically (at 200x) with closed circuit television while sperm concentration was assessed by Neubauer haemocytometer.
7] spores/mL with the aid of a Neubauer haemocytometer.
2 Traditionally the blood counts were performed manually using the haemocytometer.
Conidia in washing suspensions were counted using a haemocytometer.
Two indicators of EAC-cell activity were measured in both control and the MTX treated groups: (i) the volume of the fluids inside the peritoneal cavity; (ii) the numbers of EAC cells found in the fluid by using a haemocytometer.
Counting was then done using the counting chamber in the haemocytometer (18).
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