The 4 most common hemoglobin (Hb) variants worldwide and in the US are Hb S, Hb E, Hb C
, and Hb D.
In addition, there are other variants with electrophoretic mobilities identical or similar to those of Hb S and Hb C.
As reported previously (23), Hb O-Arab and Hb C had statistically different retention times (P <0.
4, lane 3) and slightly cathodal to Hb C on acid electrophoresis (data not shown).
Two [beta]-variants (Hb E and Hb E-Saskatoon) were identified, each of which had alkaline mobilities on the Helena Hemoglobin Electrophoresis System identical to that of Hb C (data not shown) and SPIFE mobilities (Fig.
It previously has been shown that the presence of Hb C
or S trait, both of which have substitutions at position six of the [beta] chain, can affect the accuracy of some immunoassay results (8).
Samples containing Hb C trait showed modestly higher results than the HPLC method used.
Samples with hemoglobin variants were identified by comparison of retention times on the Diamat system (Bio-Rad Clinical Laboratories) to known retention times for Hb S and Hb C.
The mean percentage of the Hb 5 in samples with Hb AS was 38% (range, 25-41%), and the mean percentage of Hb C in samples with Hb AC was 38% (range, 29-41%).
2], but not from Hb D-Punjab and Hb G-Philadelphia; Hb C and Hb O-Arab were separated from Hb A, but not completely from Hb [A.
Hb C, Hb E, and Hb O-Arab), quantification of Hb [A.