Hematoxylin


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Related to Hematoxylin: eosin, hematoxylin bodies

hematoxylin

[‚hē·mə′täk·sə·lən]
(organic chemistry)
C16H14O6 A colorless, crystalline compound occurring in hematoxylon; upon oxidation, it is converted to hematein which forms deeply colored lakes with various metals; used as a stain in microscopy.

Hematoxylin

 

a dye used in microscopy for staining plant and animal tissues. Hematoxylin is extracted by ether from the colored wood of the logwood tree, which is native to Central America and the Antilles Islands. In the course of its preparation for use in microscopy, the substance is “matured,” or oxidized to hematein, which stains cell nuclei, chromosomes, and cell membranes a blue or blue-black color.

REFERENCE

Romeis, B. Mikroskopicheskaia tekhnika. Moscow, 1953. (Translated from German.)
References in periodicals archive ?
Hematoxylin and eosin stain of cardiac biopsy showing metastatic papillary renal cell carcinoma infiltrating the myocardium, magnification 400x.
Frozen section examination of right testicular biopsy (A, hematoxylin and eosin[H&E], original magnification x 40) and its control stain (B, H&E, original magnification x 200) demonstrating dense fibrocollagenous tissue and a mixed inflammatory infiltrate with prominent capillary network.
Histologic evaluation with hematoxylin and cosin staining revealed a spindle-cell-type malignancy that originated in the dermis and extended down into the subcutaneous fat and muscle (figure 2).
2: Tumour cells presented pleomorphic, atypical hyperplasia and mitotic figures in some regions, hematoxylin and eosin x400.
The absence of hematoxylin bodies and neutrophils, the near-absence of plasma cells, and a tendency toward more widespread necrosis are important factors in distinguishing Kikuchi's disease from SLE.
Sections were then counterstained in Meyer's hematoxylin (Fisher Scientific, Pittsburgh, PA).
In general, however, sections prepared by CTPM exhibited brighter staining with eosin and stronger reaction with hematoxylin.
Hematoxylin and eosin--stained histologic sections of the lung segments showed an extensive multifocal infiltration of lymphocytes, histiocytes, and some scattered multinuclear giant cells within the framework of proliferated connective tissue and collagen fibers of the cavernous lesions.
Microscopic examination with hematoxylin and eosin staining revealed that the lesion contained large vessels with cystically dilated lumina, which were filled with erythrocytes and fibrous tissue interspersed between these vessels (figure 2).
After embedding in paraffin wax, 4-[micro]m sections were stained with hematoxylin and eosin.
One audience member agreed with this point, noting that permanent hematoxylin and eosin stains and the frozen section margins that are taken with Mohs surgery for SCC make it difficult for pathologists to identify perineural invasion.
The slides were washed as above, developed with diaminobenzidine (Dako), and counterstained with hematoxylin.